Antz

Saturday, March 17, 2007

One week has past since the start of my attachment. Hmm...felt kinda depressed during the lst few days of my attachment. Maybe its becoz of FYP (Final Year Project) and attachment. Dunoe. But Felt better on thursday and friday. Went to asked my supervisor some questions about my project, and it turns out that im doing the same thing as my senior. Well, not that bad, considering the fact that i understand the procedure (PCR, Gel electrophoresis, restriction fragment length polymorphism (RFLP) and then gel electrophoresis of RFLP products) Hmm...some doubts still lingered in my mind but i will try to clarify them. Wats more im doing this project alone so i have to be very clear on my project.

This one week of attachment has made me more independent. Being attached to a company alone is not a bad thing after all, at least it has taught to be more independent and not rely on my lab partner all the time. Besides that, i oso how to carry out PCR and gel electrophoresis on my own. Haha...realise that its not like wat we did in our school lab. In our school lab, we jus hav to aliquot the reagents into our tubes, then the lecturers and lab tech will do the PCR for us. Then the lab tech will prepare the gel for us and all we hav to do is to load the PCR products into the gel and then they will carry out the gel electrophoresis for us.

But after this first week of attachment, i found out that things are not like wat we did in school. I had to do everything by myself. I learned how to set the PCR machine according to my PCR conditions and to prepare the gel for gel electrophoresis. WOW. Cool. Haha...but the waiting time is horrible. One whole PCR cycle takes approximately 2 hours, and one gel electrophoresis takes approximately one and a half hours. Gosh.

23 more weeks of attachment to go. Jiayou le! When all this is over, im really gonna celebrate :)
Yup, so hang in there :)

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